Traditional PCR is a technique that uses a thermal cycler to amplify DNA fragments in a reaction at a set temperature. This method can be used to clone genomic DNA or RNA. Real-time PCR is similar to traditional PCR, but the amplification process is monitored in real-time. This allows for a more accurate determination of the starting point and length of the amplification reaction.
Benefits of Using Real-Time PCR
Real-time PCR system is a newer and more advanced form of PCR that offers many benefits over traditional PCR. One of the main benefits is that real-time PCR can be used to determine the exact amount of DNA or RNA present in a sample, which can be important for testing purposes. Additionally, real-time PCR is faster than traditional PCR, so it can be used to analyze samples in a hurry. Lastly, real-time PCR can be used to detect variants of genes, which is an important tool for researching genetic disorders.
Real-time PCR is different from traditional PCR in that it uses a fluorescent dye to probe the reaction mixture at regular intervals. This allows researchers to see which DNA molecules are being amplified and how much progress has been made. Additionally, real-time PCR can be used to determine the accuracy of a particular DNA sequence.
What are some applications for real-time PCR?
Real-time PCR has many potential applications in genomics , microbiology, molecular biology, and biotechnology. For example, it has been used to study the process of apoptosis (programmed cell death) in breast cancer cells as well as in differentiating stem cells. It is also being used to track disease progression in patients with cancer and other diseases including COVID-19 and AIDS. It is the best replacement for traditional PCR.